FLOW-INJECTION DETERMINATION OF 3-HYDROXYBUTYRATE IN SERUM WITH AN IMMOBILIZED 3-HYDROXYBUTYRATE DEHYDROGENASE REACTOR AND FLUORESCENCE DETECTION

被引：9

作者：

KIBA, N

KOEMADO, H

FURUSAWA, M

机构：

[1] Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu

来源：

TALANTA | 1994年 / 41卷 / 09期

关键词：

D O I：

10.1016/0039-9140(94)E0056-W

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A flow-injection system with an immobilized enzyme reactor is proposed for the determination of 3-hydroxybutyrate. 3-Hydroxybutyrate dehydrogenase is immobilized on aminated poly(vinyl alcohol) beads and packed into a stainless-steel column (4 cm x 4 mm I.D.). Serum is diluted and filtered. Sample solution (20 mul) is injected into the carrier stream [4mM NAD+ in glycine buffer (pH 9.3)]. The NADH formed is detected at 465 nm (excitation at 340 nm). The calibration graph is linear for 0.7-500muM 3-hydroxybutyrate; the detection limit is 0.5muM.

引用

页码：1583 / 1586

页数：4

共 10 条

[1] A SPECTROPHOTOMETRIC, ENZYMATIC ASSAY FOR D-3-HYDROXYBUTYRATE THAT IS NOT DEPENDENT ON HYDRAZINE [J].