STARCH DETERMINATION IN HORTICULTURAL PLANT-MATERIAL BY AN ENZYMATIC-COLORIMETRIC PROCEDURE

The starch content of horticultural plant material (including leaves and woody tissues such as roots, stems, twigs, trunks and bark) was determined by an enzymic procedure. A heat‐stable α‐amylase and amyloglucosidase were used to hydrolyse the starch in the finely ground plant material to glucose. The glucose produced was measured colorimetrically using a coupled glucose oxidase/peroxidase reaction. Conditions necessary for maximum glucose recovery were established. Plant material analysed included samples from macadamia (Macadamia integrifolia Maiden and Betche, trunk and bark), mango (Mangifera indica L; stems and roots), lychee (Litchi chinensis Sonn; leaves, stems, twigs and roots), pineapple (Ananas comosus (L) Merrill; leaves, stems and roots) and nectarine (Prunus persica (L) Batsch var nucipersica (Suckow) C K Schneid; twigs, shoots, branches, limbs, roots and trunk). Starch values ranged from less than 14% to greater than 30%. The method allows assessment of variations in the starch content of perennial plants and may be used in crop management. Copyright © 1990 John Wiley & Sons, Ltd