ESTABLISHMENT OF HUMAN MICROGLIAL CELL-LINES AFTER TRANSFECTION OF PRIMARY CULTURES OF EMBRYONIC MICROGLIAL CELLS WITH THE SV40 LARGE T-ANTIGEN

被引：222

作者：

JANABI, N ^{[1
]}

PEUDENIER, S ^{[1
]}

HERON, B ^{[1
]}

NG, KH ^{[1
]}

TARDIEU, M ^{[1
]}

机构：

[1] INSERM,U347,F-94276 LE KREMLIN BICETR,FRANCE

来源：

NEUROSCIENCE LETTERS | 1995年 / 195卷 / 02期

关键词：

HUMAN EMBRYONIC CNS; MICROGLIA; TRANSFORMED CELL LINE; CYTOKINES;

D O I：

10.1016/0304-3940(94)11792-H

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Four continuous cell lines of human microglial cells were obtained by transfection of enriched cultures of human embryonic brain derived macrophages with a plasmid encoding for the large T antigen of SV40. The transformed cells had the macrophagic characteristics of adherence and intra-cytoplasmic non-specific esterase activity. They could phagocytize zymosan particles but the phagocytic activity remained low. They expressed several macrophagic antigens but not the monocytic markers CD14, CD4, CD68/Ki-M6 and CD11c. The cells could be activated to express class II major histocompatibility complex antigens after interferon-gamma activation, Finally, interleukin-6 was produced spontaneously by the cells and this production was further increased after interleukin-1 alpha stimulation.

引用

页码：105 / 108

页数：4

共 19 条

[1] INVIVO SEQUENCE REQUIREMENTS OF THE SV40 EARLY PROMOTER REGION [J].