A POINT MUTATION WITHIN THE ATP-BINDING SITE INACTIVATES BOTH CATALYTIC FUNCTIONS OF THE ATP-DEPENDENT PROTEASE LA (LON) FROM ESCHERICHIA-COLI

被引：30

作者：

FISCHER, H ^{[1
]}

GLOCKSHUBER, R ^{[1
]}

机构：

[1] ETH ZURICH,INST MOLEK BIOL & BIOPHYS,CH-8093 ZURICH,SWITZERLAND

来源：

FEBS LETTERS | 1994年 / 356卷 / 01期

关键词：

PROTEASE LA (LON); ATP-DEPENDENT PROTEOLYSIS; WALKER A SEQUENCE; ATPASE-DEFICIENT MUTANT;

D O I：

10.1016/0014-5793(94)01244-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A point mutant in the ATP-binding motif(GPPGVGK(362)T) of the ATP-dependent protease La from Escherichia coli was investigated in which the lysine at position 362 was replaced by an alanine. The catalytic efficiency of the K362A mutant is at least two orders of magnitude lower than that of wild-type protease La due to a decreased V-max and an increased K-M for ATP. Simultaneously, the peptidase activity of La K362A is almost completely eliminated. Since selective inactivation of the peptidase activity of La does not affect its intrinsic ATPase activity, coupling of proteolysis with ATP hydrolysis is only uni-directional in this energy-dependent protease.

引用

页码：101 / 103

页数：3

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