TRANSFORMATION BY AGROBACTERIUM-RHIZOGENES AND REGENERATION OF TRANSGENIC SHOOTS OF THE WILD SOYBEAN GLYCINE-ARGYREA

被引：5

作者：

KUMAR, V ^{[1
]}

JONES, B ^{[1
]}

DAVEY, MR ^{[1
]}

机构：

[1] UNIV NOTTINGHAM, DEPT SURG, PLANT GENET MANIPULAT GRP, NOTTINGHAM NG7 2RD, ENGLAND

来源：

PLANT CELL REPORTS | 1991年 / 10卷 / 03期

关键词：

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Glycine argyrea accession G1420 was evaluated for its response to inoculation with Agrobacterium rhizogenes strains LBA9402 and A4T, carrying wild type Ri plasmids, and by strains R1601 and A4TIII with engineered plasmids. Hypocotyls from young seedlings were the most responsive in producing roots at inoculation sites. Root production was also dependent on bacterial concentration. Excised, cultured roots produced green nodular callus which regenerated shoots on SC2 medium containing 1.1 mg 1(-1) 6-benzylaminopurine and 0.005 mg 1(-1) indole-3-butyric acid. The transformed nature of the roots and of callus regenerating shoots was confirmed by the presence of opines and by dot blot analysis for Ri TL-DNA. Tissues regenerated from roots transformed by A. rhizogenes strains R1601 and A4TIII exhibited NPTII enzyme activity, confirming the stable integration and expression of the chimaeric kanamycin resistance gene in transgenic tissues.

引用

页码：135 / 138

页数：4

共 31 条

[21] GENETIC-MODIFICATION OF POTATO DEVELOPMENT USING RI T-DNA [J].

OOMS, G ;

KARP, A ;

BURRELL, MM ;

TWELL, D ;

ROBERTS, J .

THEORETICAL AND APPLIED GENETICS, 1985, 70 (04) :440-446

[22] GENOTYPIC VARIABILITY OF SOYBEAN RESPONSE TO AGROBACTERIUM STRAINS HARBORING THE TI-PLASMID OR RI-PLASMID [J].

OWENS, LD ;

CRESS, DE .

PLANT PHYSIOLOGY, 1985, 77 (01) :87-94

[23] TRANSFORMATION AND REGENERATION OF THE LEGUME LOTUS-CORNICULATUS - A SYSTEM FOR MOLECULAR STUDIES OF SYMBIOTIC NITROGEN-FIXATION [J].

PETIT, A ;

STOUGAARD, J ;

KUHLE, A ;

MARCKER, KA ;

TEMPE, J .

MOLECULAR & GENERAL GENETICS, 1987, 207 (2-3) :245-250

[24] INCREASED VIRULENCE OF AGROBACTERIUM-RHIZOGENES CONFERRED BY THE VIR REGION OF PTIBO542 - APPLICATION TO GENETIC-ENGINEERING OF POPLAR [J].

PYTHOUD, F ;

SINKAR, VP ;

NESTER, EW ;

GORDON, MP .

BIO-TECHNOLOGY, 1987, 5 (12) :1323-1327

[25] EXPRESSION OF A CHIMAERIC KANAMYCIN RESISTANCE GENE INTRODUCED INTO THE WILD SOYBEAN GLYCINE-CANESCENS USING A COINTEGRATE RI PLASMID VECTOR [J].

RECH, EL ;

GOLDS, TJ ;

HUSNAIN, T ;

VAINSTEIN, MH ;

JONES, B ;

HAMMATT, N ;

MULLIGAN, BJ ;

DAVEY, MR .

PLANT CELL REPORTS, 1989, 8 (01) :33-36

[26] HAIRY ROOT TRANSFORMATION IN ALFALFA (MEDICAGO-SATIVA L) [J].

SPANO, L ;

MARIOTTI, D ;

PEZZOTTI, M ;

DAMIANI, F ;

ARCIONI, S .

THEORETICAL AND APPLIED GENETICS, 1987, 73 (04) :523-530

[27] THE AGROBACTERIUM-RHIZOGENES PRI TL-DNA SEGMENT AS A GENE VECTOR SYSTEM FOR TRANSFORMATION OF PLANTS [J].

STOUGAARD, J ;

ABILDSTEN, D ;

MARCKER, KA .

MOLECULAR & GENERAL GENETICS, 1987, 207 (2-3) :251-255

[28] RI-PLASMID AS A HELPER FOR INTRODUCING VECTOR DNA INTO ALFALFA PLANTS [J].

SUKHAPINDA, K ;

SPIVEY, R ;

SHAHIN, EA .

PLANT MOLECULAR BIOLOGY, 1987, 8 (03) :209-216

[29] TRANSFORMATION OF SEVERAL SPECIES OF HIGHER-PLANTS BY AGROBACTERIUM-RHIZOGENES - SEXUAL TRANSMISSION OF THE TRANSFORMED GENOTYPE AND PHENOTYPE [J].

TEPFER, D .

CELL, 1984, 37 (03) :959-967

[30]

TEPFER DA, 1981, CR ACAD SCI III-VIE, V292, P153

← 1 2 3 4 →