COMPENSATING FOR INSTRUMENTAL AND SAMPLING BIASES ACCOMPANYING ELECTROKINETIC INJECTION IN CAPILLARY ZONE ELECTROPHORESIS

被引：52

作者：

LEE, TT

YEUNG, ES

机构：

[1] IOWA STATE UNIV SCI & TECHNOL,DEPT CHEM,AMES,IA 50011

[2] IOWA STATE UNIV SCI & TECHNOL,US DOE,AMES LAB,AMES,IA 50011

来源：

ANALYTICAL CHEMISTRY | 1992年 / 64卷 / 11期

关键词：

D O I：

10.1021/ac00035a007

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The quantitative precision in capillary zone electrophoresis (CZE) with electrokinetic injection can be improved by proper correction with the integrated current during injection. By monitoring the electrophoretic current during separation, one can also correct for temporal fluctuations in the analyte migration velocity. A model based on field-amplified capillary electrophoresis is used to describe the electrokinetic injection process involving sample solutions with distinct conductivities, and an equation is developed to allow normalization of peak areas at the 0-5% level without the need of quantitative internal standardization.

引用

收藏

页码：1226 / 1231

页数：6

相关论文

共 28 条

[11] ANALYSIS OF FACTORS CAUSING PEAK BROADENING IN CAPILLARY ZONE ELECTROPHORESIS [J].

HUANG, XH ;

COLEMAN, WF ;

ZARE, RN .

JOURNAL OF CHROMATOGRAPHY, 1989, 480 :95-110

[12] NONUNIFORM ELECTRICAL-FIELD EFFECT CAUSED BY DIFFERENT CONCENTRATIONS OF ELECTROLYTE IN CAPILLARY ZONE ELECTROPHORESIS [J].

HUANG, XH ;

OHMS, JI .

JOURNAL OF CHROMATOGRAPHY, 1990, 516 (01) :233-240

[13] BIAS IN QUANTITATIVE CAPILLARY ZONE ELECTROPHORESIS CAUSED BY ELECTROKINETIC SAMPLE INJECTION [J].

HUANG, XH ;

GORDON, MJ ;

ZARE, RN .

ANALYTICAL CHEMISTRY, 1988, 60 (04) :375-377

[14] QUANTITATIVE-ANALYSIS OF LOW-MOLECULAR WEIGHT CARBOXYLIC-ACIDS BY CAPILLARY ZONE ELECTROPHORESIS CONDUCTIVITY DETECTION [J].

HUANG, XH ;

LUCKEY, JA ;

GORDON, MJ ;

ZARE, RN .

ANALYTICAL CHEMISTRY, 1989, 61 (07) :766-770

[15] FACTORS THAT INFLUENCE MOBILITY, RESOLUTION, AND SELECTIVITY IN CAPILLARY ZONE ELECTROPHORESIS .1. SODIUM-PHOSPHATE VS POTASSIUM PHOSPHATE [J].

ISSAQ, HJ ;

ATAMNA, IZ ;

METRAL, CJ ;

MUSCHIK, GM .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1990, 13 (07) :1247-1259

[16] ZONE ELECTROPHORESIS IN OPEN-TUBULAR GLASS-CAPILLARIES [J].

JORGENSON, JW ;

LUKACS, KD .

ANALYTICAL CHEMISTRY, 1981, 53 (08) :1298-1302

[17] OPTIMIZATION OF SENSITIVITY AND SEPARATION IN CAPILLARY ZONE ELECTROPHORESIS WITH INDIRECT FLUORESCENCE DETECTION [J].

KUHR, WG ;

YEUNG, ES .

ANALYTICAL CHEMISTRY, 1988, 60 (23) :2642-2646

[18] FACILITATING DATA TRANSFER AND IMPROVING PRECISION IN CAPILLARY ZONE ELECTROPHORESIS WITH MIGRATION INDEXES [J].

LEE, TT ;

YEUNG, ES .

ANALYTICAL CHEMISTRY, 1991, 63 (24) :2842-2848

[19] CAPILLARY ZONE ELECTROPHORESIS - EFFECT OF PHYSICAL PARAMETERS ON SEPARATION EFFICIENCY AND QUANTITATION [J].

LUKACS, KD ;

JORGENSON, JW .

JOURNAL OF HIGH RESOLUTION CHROMATOGRAPHY & CHROMATOGRAPHY COMMUNICATIONS, 1985, 8 (08) :407-411

[20] CONCENTRATION DISTRIBUTIONS IN FREE ZONE ELECTROPHORESIS [J].

MIKKERS, FEP ;

EVERAERTS, FM ;

VERHEGGEN, TPEM .

JOURNAL OF CHROMATOGRAPHY, 1979, 169 (FEB) :1-10