CA2+ IS ESSENTIAL FOR MULTISTEP ACTIVATION OF THE HEAT-SHOCK FACTOR IN PERMEABILIZED CELLS

被引：127

作者：

PRICE, BD

CALDERWOOD, SK

机构：

[1] Joint Center Radiation Therapy, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1991年 / 11卷 / 06期

关键词：

D O I：

10.1128/MCB.11.6.3365

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a novel permeabilized-cell system to study transcription mechanisms. In permeabilized cells, heat-induced activation of the heat shock factor and transcription of the hsp70 gene require Ca2+. Activation involves at least two steps: Ca2+-and heat-dependent activation of heat shock factor binding and a second step, prior to transcription of hsp70, that requires ATP and is sensitive to genistein, a protein kinase inhibitor.

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页码：3365 / 3368

页数：4

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