HUMAN FOAMY VIRUS GENOME POSSESSES AN INTERNAL, BEL-1-DEPENDENT AND FUNCTIONAL PROMOTER

被引:142
作者
LOCHELT, M [1 ]
MURANYI, W [1 ]
FLUGEL, RM [1 ]
机构
[1] DEUTSCH KREBSFORSCHUNGSZENTRUM,FORSCH SCHWERPUNKT ANGEW TUMORVIROL,RETROVIRALE GENEXPRESS ABT,D-69009 HEIDELBERG,GERMANY
关键词
INTRAGENIC CAP SITE; SPUMARETROVIRUS; TRANSCRIPTION; TRANSACTIVATION;
D O I
10.1073/pnas.90.15.7317
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The human foamy or spumaretrovirus (HSRV) is a complex retrovirus that encodes the three retroviral genes gag, pol, and env and, in addition, at least three bel genes. The HSRV Bel-1 protein was identified as a transcriptional trans-activator. HSRV transcription starts in the 5' long terminal repeat at a defined guanine residue. We report here that a second efficiently utilized start site of transcription is contained within a HSRV env DNA sequence upstream of the bel genes. Bel-specific transcripts that initiate at the internal transcriptional start site at nt 9196 were identified in HSRV-infected cells by primer extension and S1 nuclease analysis, and the intragenic promoter was shown to be constitutively activated by Bel-1 in the HSRV provirus. In transient expression assays with indicator gene constructs, expression by the HSRV intragenic promoter/enhancer is Bel-1 dependent. The data provide evidence for an intragenic start site of transcription in the genome of a complex, exogenous human retrovirus and are discussed in terms of a model for regulating spumaretroviral gene expression.
引用
收藏
页码:7317 / 7321
页数:5
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