MYOSIN IN ONION (ALLIUM-CEPA) BULB SCALE EPIDERMAL-CELLS - INVOLVEMENT IN DYNAMICS OF ORGANELLES AND ENDOPLASMIC-RETICULUM

被引:49
作者
LIEBE, S
QUADER, H
机构
[1] Fakultät Für Biologie, Univ. Heidelberg, Heidelberg, D-69120
关键词
ACTIN FILAMENTS; ALLIUM-CEPA; ER; IMMUNOCYTOCHEMISTRY; MYOSIN; NEM; ONION;
D O I
10.1111/j.1399-3054.1994.tb02200.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Studied with the fluorochrome 3,3-dihexyloxacarbocyanine iodide [(DIOC6(3)], the dynamic system of the endoplasmic reticulum (ER) in epidermal cells of onion bulb scales consists of long, tubular strands moving together with organelles in the deeper cytoplasm, and of a less mobile network composed of tubular and lamellar elements at the cell periphery. Treatment with the sulfhydryl-reagent N-ethylmaleimide (NEM) inhibited organelle and ER movement, and caused the fusion of ER-tubules into flat sheets. Fixed, long, tubular ER strands were formed by lowering the cytosolic pH of NEM-treated cells. Both these observations indicate the involvement of myosin in the dynamics of organelles and ER. Using a monoclonal antibody against murine skeletal muscle myosin (known to cross-react with plant myosin; Tang et al. 1989, J. Cell Sci. 92: 569-574), myosin was identified by immunofluorescence microscopy. Mapping the distribution of myosin, actin filaments, ER, and organelles in different phases of recovery after centrifugation of epidermal cells, co-localization of myosin with ER and organelles but not with actin filaments was observed, supporting the hypothesis that a membrane bound motor protein exists in onion epidermal cells, which translocates organelles and the endoplasmic reticulum along actin filaments.
引用
收藏
页码:114 / 124
页数:11
相关论文
共 40 条
[21]   ACTIN-ENDOPLASMIC RETICULUM COMPLEXES IN DROSERA - THEIR STRUCTURAL RELATIONSHIP WITH THE PLASMALEMMA, NUCLEUS, AND ORGANELLES IN CELLS PREPARED BY HIGH-PRESSURE FREEZING [J].
LICHTSCHEIDL, IK ;
LANCELLE, SA ;
HEPLER, PK .
PROTOPLASMA, 1990, 155 (1-3) :116-126
[22]  
LICHTSCHEIDL IK, 1988, EUR J CELL BIOL, V46, P367
[23]   LOWERING PH IN BLOOD-PLATELETS DISSOCIATES MYOSIN PHOSPHORYLATION FROM SHAPE CHANGE AND MYOSIN ASSOCIATION WITH THE CYTOSKELETON [J].
NACHMIAS, VT ;
YOSHIDA, K ;
GLENNON, C .
JOURNAL OF CELL BIOLOGY, 1987, 105 (04) :1761-1769
[24]   MECHANISM OF INHIBITION OF RELAXATION BY N-ETHYLMALEIMIDE TREATMENT OF MYOSIN [J].
PEMRICK, S ;
WEBER, A .
BIOCHEMISTRY, 1976, 15 (23) :5193-5198
[25]  
PESACRETA T C, 1991, Plant Physiology (Rockville), V96, P129
[26]  
QIAO L, 1989, Cell Biology International Reports, V13, P107, DOI 10.1016/S0309-1651(89)80012-8
[27]  
QUADER H, 1987, EUR J CELL BIOL, V44, P17
[28]   THE CYTOSKELETON OF COBAEA SEED HAIRS - PATTERNING DURING CELL-WALL DIFFERENTIATION [J].
QUADER, H ;
DEICHGRABER, G ;
SCHNEPF, E .
PLANTA, 1986, 168 (01) :1-10
[29]   INFLUENCE OF CYTOSOLIC PH CHANGES ON THE ORGANIZATION OF THE ENDOPLASMIC-RETICULUM IN EPIDERMAL-CELLS OF ONION BULB SCALES - ACIDIFICATION BY LOADING WITH WEAK ORGANIC-ACIDS [J].
QUADER, H ;
FAST, H .
PROTOPLASMA, 1990, 157 (1-3) :216-224
[30]   ENDOPLASMIC-RETICULUM AND CYTOPLASMIC STREAMING - FLUORESCENCE MICROSCOPIC OBSERVATIONS IN ADAXIAL EPIDERMIS CELLS OF ONION BULB SCALES [J].
QUADER, H ;
SCHNEPF, E .
PROTOPLASMA, 1986, 131 (03) :250-252