EXPRESSION IN ESCHERICHIA-COLI OF A CLAVAMINIC ACID SYNTHASE ISOZYME - A TRIFUNCTIONAL OXYGENASE INVOLVED IN CLAVULANIC ACID BIOSYNTHESIS

被引：24

作者：

LAWLOR, EJ

ELSON, SW

HOLLAND, S

CASSELS, R

HODGSON, JE

LLOYD, MD

BALDWIN, JE

SCHOFIELD, CJ

机构：

[1] DYSON PERRINS LAB,OXFORD OX1 3QY,ENGLAND

[2] OXFORD CTR MOLEC SCI,OXFORD OX1 3QY,ENGLAND

来源：

TETRAHEDRON | 1994年 / 50卷 / 29期

关键词：

D O I：

10.1016/S0040-4020(01)85347-3

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Purification and sequencing of clavaminic acid synthase (CAS) activities from S. clavuligerus SC2 provided evidence for the existence of two CAS isozymes. One of these isozymes was cloned and expressed in E. coli. The recombinant CAS isozyme was shown to catalyse the hydroxylation of (2S)-5-guanidino-2-(2'-oxoazetidin-1'-yl)pentanoic acid, in addition to the production of clavaminic acid from dihydroclavaminic acid via proclavaminic acid consistent with the trifunctional role proposed for clavaminic acid synthase in the biosynthesis of clavulanic acid. A preliminary purification and characterisation of the recombinant isozyme is reported.

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页码：8737 / 8748

页数：12

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