EPSTEIN-BARR-VIRUS LATENT MEMBRANE PROTEIN-2A BLOCKS CALCIUM MOBILIZATION IN B-LYMPHOCYTES

被引：118

作者：

MILLER, CL

LONGNECKER, R

KIEFF, E

机构：

[1] HARVARD UNIV,SCH MED,DEPT MED,75 FRANCIS ST,BOSTON,MA 02115

[2] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET,BOSTON,MA 02115

来源：

JOURNAL OF VIROLOGY | 1993年 / 67卷 / 06期

关键词：

D O I：

10.1128/JVI.67.6.3087-3094.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

LMP2A is expressed in latent Epstein-Barr virus (EBV) infection and interacts with LMP1 and members of the src tyrosine kinase family in the plasma membrane. Since tyrosine kinases mediate receptor-induced changes in intracellular free calcium, the effect of LMP2A on receptor-mediated intracellular calcium mobilization was evaluated by stably expressing LMP2A in an EBV-negative Burkitt tumor cell line (BJAB) or in LMP1-converted BJAB cells. LMP2A significantly blocked calcium mobilization following class II, CD19, or immunoglobulin M cross-linking. LMP2A effects were partially reversed in LMP1-converted cell lines. These results are compatible with LMP2A acting in latent B-lymphocyte infection to downmodulate LMP1 effects on cell growth or to inhibit induction of lytic EBV infection in specific human tissues following receptor ligation.

引用

页码：3087 / 3094

页数：8

共 58 条

[21] STIMULATION OF PROTEIN TYROSINE PHOSPHORYLATION BY THE LYMPHOCYTE-B ANTIGEN RECEPTOR [J].