A protein complex from Choristoneura fumiferana gut-juice involved in the precipitation of delta-endotoxin from Bacillus thuringiensis subsp sotto

被引：27

作者：

Milne, RE ^{[1
]}

Pang, ASD ^{[1
]}

Kaplan, H ^{[1
]}

机构：

[1] UNIV OTTAWA, DEPT CHEM, OTTAWA, ON K1N 6N5, CANADA

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1995年 / 25卷 / 10期

关键词：

Bacillus thuringiensis; Choristoneura fumiferana; delta-endotoxin; gut proteins; protein precipitation;

D O I：

10.1016/0965-1748(95)00046-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 75 kDa protein from spruce budworm (Choristoneura fumiferana) gut-juice has been isolated and shown to cause a specific precipitation of the delta-endotoxin from Bacillus thurirtgiensis subsp, soffo. This 75 kDa protein, separated by either column chromatography or SDS-PAGE, caused precipitation of the sotto toxin in both agarose diffusion gels and the PAGE gels, The precipitation event leads to limited proteolysis of the toxin and loss of larval toxicity, SDSPAGE analysis of the precipitated toxin indicates that proteolysis of the toxin is not a prerequisite for precipitation, The protein responsible for precipitation, exhibits elastase-like adtivity and appears to be a complex which partially dissociates during boiling in SDS-PAGE sample buffer, Gut-juice from gypsy moth, forest tent caterpillar and white mark tussock moth also precipitated 6-endotoxin, but silkworm gut-juice gave a much weaker response, These results provide further evidence that, in the larval gut, differential processing of 6-endotoxin may play a role in the expression of activity towards various insect larvae,

引用

页码：1101 / 1114

页数：14

共 32 条

[11] MIDGUT PROTEASE ACTIVITIES IN 12 PHYTOPHAGOUS LEPIDOPTERAN LARVAE - DIETARY AND PROTEASE INHIBITOR INTERACTIONS [J].