DEVELOPMENT OF A NEEDLE TYPE GLUCOSE BIOSENSOR

A needle type glucose biosensor has been developed for monitoring of glucose levels during hemorrhagic shock. The sensor employs polycarbonate membranes that are either untreated or treated with Nafion or Silastic, and Glucose Oxidase enzyme which is immobilized on modified carbon powder in a gel matrix. Sensor performance was evaluated in vitro and the sensor showed a sensitivity of up to 10 nA/mM and a linear range of up to 30 mM. Evaluation of the sensor response in serum showed similar sensitivity and linear range as obtained using calibration curves in buffer solution. The sensors were operated continuously for 28 days in phosphate buffer solution and no significant change in the sensitivity and the linear range was observed during the first 7 days. Sensors show a minimum change in their performance when stored inactive in buffer at 4 degrees C for at least eight weeks.