IMAGING ADHESION FORCES AND ELASTICITY OF LYSOZYME ADSORBED ON MICA WITH THE ATOMIC-FORCE MICROSCOPE

被引：236

作者：

RADMACHER, M ^{[1
]}

FRITZ, M ^{[1
]}

CLEVELAND, JP ^{[1
]}

WALTERS, DA ^{[1
]}

HANSMA, PK ^{[1
]}

机构：

[1] UNIV CALIF SANTA BARBARA,DEPT PHYS,SANTA BARBARA,CA 93106

来源：

LANGMUIR | 1994年 / 10卷 / 10期

关键词：

D O I：

10.1021/la00022a068

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have investigated the adsorption of lysozyme molecules on mica substrates in buffer by atomic force microscopy (AFM). The stability of the adsorbate is influenced by buffer conditions. At pH 4 single molecules can be imaged stably, while at pH 6.4 only aggregates of molecules are observed. Maps of the tip-sample interaction were obtained by recording laterally resolved force curves. These force curves showed that the adhesion of the tip on the lysozyme is smaller than on the mica. The intrinsic viscoelastic properties of lysozyme could be calculated by analyzing these force curves.

引用

页码：3809 / 3814

页数：6

共 30 条

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