EFFECTS OF SOLVENT MOLECULAR TOXICITY AND MICROENVIRONMENT COMPOSITION ON THE DELTA-DEHYDROGENATION ACTIVITY OF ARTHROBACTER-SIMPLEX CELLS

被引：25

作者：

PINHEIRO, HM

CABRAL, JMS

机构：

[1] Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, Lisbon

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1991年 / 37卷 / 02期

关键词：

D O I：

10.1002/bit.260370202

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Solvent selection tests were carried out for the DELTA1 dehydrogenation of 6-alpha-methylhydrocortisone-21-acetate by Arthrobacter simplex cells in the presence of organic solvents. Solubility limits were determined for substrate and product in dry and water-saturated solvents and solvent mixtures. Molecular toxicity levels were estimated by measuring the dehydrogenation activity decay of freely suspended cells incubated in solvent-saturated aqueous media. Chloroform and n-decan-1-ol were the best choice of solvent, for both solubility and catalytic stability. High concentrations of water-soluble additives, such as monosodium glutamate, were found to greatly improve the retention of activity in chloroform-saturated media.

引用

页码：97 / 102

页数：6

共 19 条

[11] PERMEATION OF HYDROCORTISONE AND HYDROCORTISONE-21-ALKYL ESTERS THROUGH SILICONE-RUBBER MEMBRANES - RELATIONSHIP TO REGULAR SOLUTION SOLUBILITY BEHAVIOR [J].