OPTIMIZATION OF CULTURE CONDITIONS FOR PRODUCTION OF EICOSAPENTAENOIC ACID BY MORTIERELLA-ELONGATA NRRL-5513

被引：23

作者：

BAJPAI, PK ^{[1
]}

BAJPAI, P ^{[1
]}

WARD, OP ^{[1
]}

机构：

[1] UNIV WATERLOO,DEPT BIOL,WATERLOO N2L 3G1,ONTARIO,CANADA

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1992年 / 9卷 / 01期

关键词：

EICOSAPENTAENOIC ACID; MORTIERELLA-ELONGATA; OMEGA-3 FATTY ACIDS;

D O I：

10.1007/BF01576363

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

When Mortierella elongata NRRL 5513 was cultured in shake flasks at 25-degrees-C, mycelial growth reached a stationary phase at 48 h but maximum eicosapentaenoic acid (EPA) production was observed at 6 days. When incubated at 11-degrees-C, EPA production also continued to rise during the stationary phase of growth, reaching a maximum after 10 days. An initial culture pH of 6.1 was found to be optimum for EPA production. The effect of temperature on EPA production was dependent on medium constituents. In glucose and linseed oil supplemented media, optimum temperature for EPA production was 11 and 15-degrees-C respectively. A maximum EPA yield of 0.61 g/l was obtained in linseed oil (2%), yeast extract (0.5%) supplemented basal medium. Maximum EPA content as a percentage of lipids (15.12%) was observed when the latter medium was supplemented with 0.25% urea.

引用

页码：11 / 17

页数：7

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