DIFFERENTIAL TARGETING OF PROTEIN-KINASE-C AND CAM KINASE-II SIGNALINGS TO VIMENTIN

被引:71
作者
OGAWARA, M
INAGAKI, N
TSUJIMURA, K
TAKAI, Y
SEKIMATA, M
HA, MH
IMAJOHOHMI, S
HIRAI, S
OHNO, S
SUGIURA, H
YAMAUCHI, T
INAGAKI, M
机构
[1] TOKYO METROPOLITAN INST GERONTOL, DEPT NEUROPHYSIOL, ITABASHI KU, TOKYO 173, JAPAN
[2] UNIV TOKYO, INST MED SCI, MINATO KU, TOKYO 108, JAPAN
[3] YOKOHAMA CITY UNIV, SCH MED, DEPT BIOL MOLEC, KANAZAWA KU, YOKOHAMA, KANAGAWA 236, JAPAN
[4] TOKYO METROPOLITAN INST NEUROSCI, DEPT MOLEC NEUROBIOL, FUCHU, TOKYO 183, JAPAN
[5] UNIV TOKUSHIMA, FAC PHARMACEUT SCI, TOKUSHIMA 770, JAPAN
[6] UNIV TOKYO, SCH MED, DEPT OBSTET & GYNECOL, BUNKYO KU, TOKYO 113, JAPAN
关键词
D O I
10.1083/jcb.131.4.1055
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Hydrolysis of inositol phospholipids by receptor stimulation activates two separate signaling pathways, one leading to the activation of protein kinase C (C kinase) via formation of diacylglycerol. The other is the inositol trisphosphate (IP3)/Ca2+ pathway and a major downstream kinase which is activated is Ca2+/calmodulin-dependent protein kinase II (CaM kinase II). To examine signaling pathways of C kinase and CaM kinase II to the cytoskeletal protein vimentin, we prepared monoclonal antibodies YT33 and MO82 which recognize the phosphorylation state of vimentin by C kinase and by CaM kinase II, respectively. Ectopic expression of constitutively active C kinase or CaM kinase II in primary cultured astrocytes by microinjection of the corresponding expression vectors induced phosphorylation of vimentin at each specific phosphorylation site, followed by reorganization of vimentin filament networks. In contrast, simultaneous activation of C kinase and CaM kinase II by inositol. phospholipid hydrolysis with receptor stimulation led to an exclusive phosphorylation of vimentin at the CaM kinase II site, not at the site of C kinase. These results indicate that the intracellular targeting of C kinase and CaM kinase II signalings to vimentin is regulated separately, under physiological conditions.
引用
收藏
页码:1055 / 1066
页数:12
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