PREPARATION AND EVALUATION OF POLYACRYLATE-COATED FUSED-SILICA CAPILLARIES FOR REVERSED-PHASE OPEN-TUBULAR LIQUID-CHROMATOGRAPHY

被引：35

作者：

SWART, R ^{[1
]}

KRAAK, JC ^{[1
]}

POPPE, H ^{[1
]}

机构：

[1] UNIV AMSTERDAM,ANALYT CHEM LAB,1018 WV AMSTERDAM,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 670卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)80277-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

By in situ photopolymerization of acrylates, thick polyacrylate films can be immobilized in 10-mu m fused-silica capillaries. Up to 1.9-mu m thick films can be produced in 0.3-1.2 m long capillaries, resulting in phase ratios >1.0 and leading to columns with high mass loadability. Owing to the high mass loadability, application of ''on-column'' UV detection is possible. The effect of incorporating alkyl acrylates in the film on retention behaviour and efficiency was extensively investigated. The success rate of the preparation of silicone-ethylhexyl acrylate films is almost 100%. The polyacrylate films are stable towards basic solutions up to pH 12. The column efficiency is demonstrated with separations of anthracene derivatives. A fast separation of methyl-substituted benzenes with on-column UV detection is shown.

引用

页码：25 / 38

页数：14

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