PROTEINS AND ANTIGENS OF PASTEURELLA-MULTOCIDA SEROTYPE-1 FROM FOWL CHOLERA

被引：19

作者：

IRELAND, L ^{[1
]}

ADLER, B ^{[1
]}

MILNER, AR ^{[1
]}

机构：

[1] MONASH UNIV,DEPT MICROBIOL,CLAYTON,VIC 3168,AUSTRALIA

来源：

VETERINARY MICROBIOLOGY | 1991年 / 27卷 / 02期

关键词：

D O I：

10.1016/0378-1135(91)90009-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The protein profiles of Pasteurella multocida serotype 1 isolates and the response of chickens to serotype 1 antigens were investigated using SDS-PAGE. Patterns obtained with Coomassie blue staining of soluble protein extracts were similar. The major difference between isolates was the position of one of the major proteins in the 34-38 kDa region. When chickens were experimentally infected with a clinical isolate of P. multocida serotype 1 various proteins were recognised by immunoblotting, including one with a relative molecular weight of 34 kDa; however, no reactions were observed in the region where LPS is known to migrate. When these infection sera were used in an EIA with purified LPS obtained from Heddleston serotype 1 type strain (X-73) they reacted strongly. Serum used for serotyping isolates in the gel diffusion precipitin test recognised many antigens in common with sera from infected birds, but some antigens were specific to typing sera.

引用

页码：175 / 185

页数：11

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[31] AN IMMUNOLOGICAL STUDY OF PASTEURELLA-SEPTICA [J].