SYNTHESIS OF CHOLESTERYL SUPPORTS AND PHOSPHORAMIDITE FOR AUTOMATED DNA-SYNTHESIS OF TRIPLE-HELIX FORMING OLIGONUCLEOTIDES (TFOS)

被引：17

作者：

VU, H ^{[1
]}

SINGH, P ^{[1
]}

LEWIS, L ^{[1
]}

ZENDEGUI, JG ^{[1
]}

JAYARAMAN, K ^{[1
]}

机构：

[1] TRIPLEX PHARMACEUT CORP,9391 GROGANS MILL RD,THE WOODLANDS,TX 77380

来源：

NUCLEOSIDES & NUCLEOTIDES | 1993年 / 12卷 / 08期

关键词：

D O I：

10.1080/07328319308018556

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DMT-Cholesteryl succinylamino solid supports (CPG, loading: 33 mumole/gram and TentaGel loading: 152 mumole/gram) and DMT-cholesteryl phosphoramidite were prepared for use in automated DNA synthesis of cholesteryl modified TFOs in the synthesis scales from 0.2 to 300 mumole. The modified TFOs were found to have a 5 to 50 fold increase in their uptake properties.

引用

页码：853 / 864

页数：12

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