CLONING OF CDNAS ENCODING THE 160 KDA SUBUNIT OF THE BOVINE CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR

被引：42

作者：

JENNY, A ^{[1
]}

KELLER, W ^{[1
]}

机构：

[1] UNIV BASEL,DEPT CELL BIOL,BIOZENTRUM,CH-4056 BASEL,SWITZERLAND

来源：

NUCLEIC ACIDS RESEARCH | 1995年 / 23卷 / 14期

关键词：

D O I：

10.1093/nar/23.14.2629

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

3'-processing of mRNA precursors depends on several protein factors. One of them, cleavage and polyadenylation specificity factor (CPSF) is required for the cleavage of the mRNA precursor as well as for the tail elongation reaction, We have obtained complementary DNA encoding the 160 kDa subunit, which had previously been shown to interact with the AAUAAA polyadenylation signal. The cDNAs code for an open reading frame of 1444 amino acids, The translated protein has a calculated molecular weight of 161 kDa and a predicted pi of 6.2. Polyclonal antibodies raised against a bacterially expressed fragment of the cDNA recognise the 160 kDa subunit of purified calf thymus CPSF. The sequence contains a possible nuclear localisation signal but none of the known RNA binding motifs, It does, however, show sequence similarities to a UV-damaged DNA binding protein (UVdDb.,

引用

页码：2629 / 2635

页数：7