DETERMINATION OF FREE MYOINOSITOL IN MILK AND INFANT FORMULA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：37

作者：

INDYK, HE ^{[1
]}

WOOLLARD, DC ^{[1
]}

机构：

[1] MINIST AGR & FISHERIES,LYNFIELD FOOD SERV CTR,AUCKLAND,NEW ZEALAND

来源：

ANALYST | 1994年 / 119卷 / 03期

关键词：

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; MYOINOSITOL; MILK; INFANT FORMULA; PREMIX;

D O I：

10.1039/an9941900397

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method is described for the determination of supplemental myo-inositol in infant formulas based on cows' milk. The technique incorporates pre-column derivatization with phenylisocyanate followed by reversed-phase gradient chromatography and ultraviolet detection. The protocol was also applied to a survey of free inositol at endogenous levels in the milk of cows, goats and humans. Nutritional preparations containing inositol at pharmacological levels are also amenable to a simplified isocratic analysis.

引用

页码：397 / 402

页数：6

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