COMPARISON OF POSTCOLUMN FLUORESCENCE DERIVATIZATION AND EVAPORATIVE LIGHT-SCATTERING DETECTION TO ANALYZE SACCHARIDES SELECTIVELY BY LC

被引：10

作者：

COQUET, A

VEUTHEY, JL

HAERDI, W

机构：

[1] Department of Inorganic, Analytical and Applied Chemistry, Sciences II, University of Geneva, Geneva 4, 1211

来源：

CHROMATOGRAPHIA | 1992年 / 34卷 / 11-12期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; POSTCOLUMN DERIVATIZATION; LIGHT-SCATTERING DETECTION; CARBOHYDRATES;

D O I：

10.1007/BF02269879

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of post-column fluorescence derivatization and evaporative light-scattering detection to analyse saccharides selectively by LC was investigated. In order to compare the performances of these two detection systems, factors such as reproducibility, detection limits and linear calibrations were determined. Despite the derivatization reaction step, post-column derivatization is superior from the standpoint of sensitivity. The detection of saccharides in beverages is also described.

引用

页码：651 / 654

页数：4

共 13 条

[1] SEPARATION OF MONOSACCHARIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - COMPARISON OF ULTRAVIOLET AND REFRACTIVE-INDEX DETECTION [J].