MICE LACKING TDT - MATURE ANIMALS WITH AN IMMATURE LYMPHOCYTE REPERTOIRE

被引：364

作者：

GILFILLAN, S ^{[1
]}

DIERICH, A ^{[1
]}

LEMEUR, M ^{[1
]}

BENOIST, C ^{[1
]}

MATHIS, D ^{[1
]}

机构：

[1] INST CHIM BIOL,INSERM,U184,UNITE BIOL MOLEC,F-67085 STRASBOURG,FRANCE

来源：

SCIENCE | 1993年 / 261卷 / 5125期

关键词：

D O I：

10.1126/science.8356452

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In adult animals, template-independent (or N) nucleotides are frequently added during the rearrangement of variable (V), diversity (D), and joining (J) segments of lymphocyte receptor genes, greatly enhancing junctional diversity. Receptor genes from adult mice carrying a mutation in the terminal deoxynucleotidyl transferase (TdT) gene have few N nucleotides, providing proof that this enzyme is essential for creating diversity. Unlike those from normal adults, receptor genes from adult mutant mice show extensive evidence of homology-directed recombination, suggesting that TdT blocks this process. Thus, switch-on of the TdT gene during the first week after birth provokes an even greater expansion of lymphocyte receptor diversity than had previously been thought.

引用

页码：1175 / 1178

页数：4

共 37 条

[21]

GILFILLAN S, UNPUB

[22] SEQUENCE HOMOLOGIES, N-SEQUENCE INSERTION AND JH GENE UTILIZATION IN VHDJH JOINING - IMPLICATIONS FOR THE JOINING MECHANISM AND THE ONTOGENIC TIMING OF LY1-B CELL AND B-CLL PROGENITOR GENERATION [J].

GU, H ;

FORSTER, I ;

RAJEWSKY, K .

EMBO JOURNAL, 1990, 9 (07) :2133-2140

[23] ONLY DFL16, DSP2, AND DQ52 GENE FAMILIES EXIST IN MOUSE IMMUNOGLOBULIN HEAVY-CHAIN DIVERSITY GENE LOCI, OF WHICH DFL16 AND DSP2 ORIGINATE FROM THE SAME PRIMORDIAL DH GENE [J].

ICHIHARA, Y ;

HAYASHIDA, H ;

MIYAZAWA, S ;

KUROSAWA, Y .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1989, 19 (10) :1849-1854

[24] SELECTION OF GAMMA-DELTA-T-CELLS WITH CANONICAL T-CELL ANTIGEN RECEPTORS IN FETAL THYMUS [J].

ITOHARA, S ;

TONEGAWA, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (20) :7935-7938

[25] T-CELL RECEPTOR DELTA-GENE MUTANT MICE - INDEPENDENT GENERATION OF ALPHA-BETA T-CELLS AND PROGRAMMED REARRANGEMENTS OF GAMMA-DELTA TCR GENES [J].

ITOHARA, S ;

MOMBAERTS, P ;

LAFAILLE, J ;

IACOMINI, J ;

NELSON, A ;

CLARKE, AR ;

HOOPER, ML ;

FARR, A ;

TONEGAWA, S .

CELL, 1993, 72 (03) :337-348

[26] 3 LYMPHOID-SPECIFIC FACTORS ACCOUNT FOR ALL JUNCTIONAL DIVERSITY CHARACTERISTIC OF SOMATIC ASSEMBLY OF T-CELL RECEPTOR AND IMMUNOGLOBULIN GENES [J].

KALLENBACH, S ;

DOYEN, N ;

DANDON, MF ;

ROUGEON, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (07) :2799-2803

[27] LACK OF N-REGIONS IN ANTIGEN RECEPTOR VARIABLE REGION GENES OF TDT-DEFICIENT LYMPHOCYTES [J].

KOMORI, T ;

OKADA, A ;

STEWART, V ;

ALT, FW .

SCIENCE, 1993, 261 (5125) :1171-1175

[28] POSITIVE SELECTION OF GAMMA-DELTA T-CELLS [J].

LAFAILLE, JJ ;

HAAS, W ;

COUTINHO, A ;

TONEGAWA, S .

IMMUNOLOGY TODAY, 1990, 11 (03) :75-78

[29] JUNCTIONAL SEQUENCES OF T-CELL RECEPTOR GAMMA-DELTA-GENES - IMPLICATIONS FOR GAMMA-DELTA-T-CELL LINEAGES AND FOR A NOVEL INTERMEDIATE OF V-(D)-J JOINING [J].

LAFAILLE, JJ ;

DECLOUX, A ;

BONNEVILLE, M ;

TAKAGAKI, Y ;

TONEGAWA, S .

CELL, 1989, 59 (05) :859-870

[30] INCREASED FREQUENCY OF N-REGION INSERTION IN A MURINE PRE-B-CELL LINE INFECTED WITH A TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE RETROVIRAL EXPRESSION VECTOR [J].

LANDAU, NR ;

SCHATZ, DG ;

ROSA, M ;

BALTIMORE, D .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (09) :3237-3243

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