CDNA SEQUENCE IDENTITY OF A VITAMIN-D-DEPENDENT CALCIUM-BINDING PROTEIN IN THE CHICK TO CALBINDIN-D-9K

被引：16

作者：

ZANELLO, SB ^{[1
]}

BOLAND, RL ^{[1
]}

NORMAN, AW ^{[1
]}

机构：

[1] UNIV NACL SUR, DEPT BIOL, RA-8000 BAHIA BLANCA, ARGENTINA

来源：

ENDOCRINOLOGY | 1995年 / 136卷 / 06期

关键词：

D O I：

10.1210/en.136.6.2784

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

1,25-dihydroxy-vitamin D-3 [1,25(OH)(2)D-3] is a steroid hormone that modulates the expression of specific proteins by a genomic mechanism of action. Calbindin D-9K is a calcium-binding protein that heretofore has only been found in mammalian tissues and whose gene expression is regulated by 1,25(OH)(2)D-3 in a tissue specific fashion. By combined reverse transcription and polymerase chain reaction, calbindin D-9K gene expression was demonstrated for the first time to be present in several chicken tissues. Subcloning and sequencing of a partial 160 bp-cDNA PCR product revealed that the cDNA corresponds to calbindin D-9K-cDNA. This constitutes the first evidence of calbindin D-9K gene presence and expression in the avian class.

引用

页码：2784 / 2787

页数：4

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