OBSERVATION OF NONCOVALENT COMPLEXES TO THE AVIDIN TETRAMER BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY

被引：58

作者：

SCHWARTZ, BL ^{[1
]}

LIGHTWAHL, KJ ^{[1
]}

SMITH, RD ^{[1
]}

机构：

[1] PACIFIC NW LAB, DEPT CHEM SCI, CHEM METHODS & SEPARAT GRP, RICHLAND, WA 99352 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 1994年 / 5卷 / 03期

关键词：

D O I：

10.1016/1044-0305(94)85034-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Intact avidin-biotin and avidin-biotin maleimide noncovalent complexes have been observed by electrospray ionization mass spectrometry (ESI-MS) by using an extended mass range quadrupole mass spectrometer. By utilizing mild ESI interface conditions, the expected solution behavior of four biotin or biotin maleimide molecules noncovalently binding to each avidin tetramer can be preserved in the gas phase. The ESI-MS results show the appropriate mass additions of 973 +/- 60 Da for biotin and 1802 +/- 40 Da for biotin maleimide to the avidin tetramer species. These results support the hypothesis that substantial retention of higher order structure is possible in the gas phase by using gentle ESI conditions.

引用

页码：201 / 204

页数：4

共 24 条

[1] DIRECT OBSERVATION OF A TERNARY COMPLEX BETWEEN THE DIMERIC ENZYME HIV-1 PROTEASE AND A SUBSTRATE-BASED INHIBITOR
BACA, M
KENT, SBH
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (10) : 3992 - 3993
[2] Bayer E A, 1980, Methods Biochem Anal, V26, P1
[3] 3-(N-MALEIMIDO-PROPIONYL) BIOCYTIN - A VERSATILE THIOL-SPECIFIC BIOTINYLATING REAGENT
BAYER, EA
ZALIS, MG
WILCHEK, M
[J]. ANALYTICAL BIOCHEMISTRY, 1985, 149 (02) : 529 - 536
[4] AN ELECTROSPRAY-IONIZATION MASS-SPECTROMETER WITH NEW FEATURES
CHOWDHURY, SK
KATTA, V
CHAIT, BT
[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1990, 4 (03) : 81 - 87
[5] DELANGE RJ, 1971, J BIOL CHEM, V246, P698
[6] ELECTROSPRAY IONIZATION-PRINCIPLES AND PRACTICE
FENN, JB
MANN, M
MENG, CK
WONG, SF
WHITEHOUSE, CM
[J]. MASS SPECTROMETRY REVIEWS, 1990, 9 (01) : 37 - 70
[7] OBSERVATION OF NONCOVALENT ENZYME SUBSTRATE AND ENZYME PRODUCT COMPLEXES BY ION-SPRAY MASS-SPECTROMETRY
GANEM, B
LI, YT
HENION, JD
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (20) : 7818 - 7819
[8] DETECTION OF NONCOVALENT RECEPTOR LIGAND COMPLEXES BY MASS-SPECTROMETRY
GANEM, B
LI, YT
HENION, JD
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (16) : 6294 - 6296
[9] GANEM B, 1991, CHEM TRACTS ORG CHEM, V6, P1
[10] MASS-SPECTROMETRIC DETECTION OF THE NONCOVALENT GDP-BOUND CONFORMATIONAL STATE OF THE HUMAN H-RAS PROTEIN
GANGULY, AK
PRAMANIK, BN
TSARBOPOULOS, A
COVEY, TR
HUANG, E
FUHRMAN, SA
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (16) : 6559 - 6560

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