HYDROXYSTEARIC ACID EFFECTS ON CDC2 HISTONE H1 KINASE-ACTIVITY IN C108 CARCINOMA-CELLS

被引：10

作者：

CASALI, E

GESMUNDO, N

FARRUGGIA, G

SPISNI, A

MASOTTI, L

机构：

[1] UNIV BOLOGNA, DIPARTMENTO BIOCHIM G MORUZZI, I-40126 BOLOGNA, ITALY

[2] UNIV PARMA, INST BIOL CHEM, I-43100 PARMA, ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 203卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.2338

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HSA at appropriate concentrations shows cytostatic and/or cytotoxic effects on murine Lewis carcinoma cell line C108. The cytostatic effect is mediated by an arrest in the cell cycle machinery, with accumulation of cells in G2-M. The combination of enzymatic assays, cell cycle kinetics studies and immunoprecipitation shows that HSA causes to a certainty an accumulation of cells in the M phase, while a similar effect in G2 has still to be demonstrated. It also inhibits histone H1 kinase activity up to 95% of that of mitotic cells, having as a direct or indirect target the cdc2 complex. (C) 1994 Academic Press, Inc.

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收藏

页码：1385 / 1391

页数：7

相关论文

共 22 条

[1] SUPEROXIDE-DISMUTASE CONTENT AND MICROSOMAL LIPID-COMPOSITION OF TUMORS WITH DIFFERENT GROWTH-RATES [J].

BARTOLI, GM ;

BARTOLI, S ;

GALEOTTI, T ;

BERTOLI, E .

BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 620 (02) :205-211

[2] GROWTH-RELATED LIPID PEROXIDATION IN TUMOR MICROSOMAL-MEMBRANES AND MITOCHONDRIA [J].

BARTOLI, GM ;

GALEOTTI, T .

BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 574 (03) :537-541

[3] CA-2+/PHOSPHOLIPID-DEPENDENT PROTEIN-KINASE ACTIVITY CORRELATES TO THE ABILITY OF TRANSFORMED LIVER-CELLS TO PROLIFERATE IN CA-2+-DEFICIENT MEDIUM [J].

BOYNTON, AL ;

WHITFIELD, JF ;

KLEINE, LP .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1983, 115 (01) :383-390

[4]

CASALI E, 1992, RECENT ADVANCES IN CELLULAR AND MOLECULAR BIOLOGY, VOL 6, V6, P245

[5] IDENTIFICATION OF THE PEROXIDATION PRODUCT HYDROXYSTEARIC ACID IN LEWIS LUNG-CARCINOMA CELLS [J].

CAVALLI, G ;

CASALI, E ;

SPISNI, A ;

MASOTTI, L .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 178 (03) :1260-1265

[6] ACTIVATION OF CDC2 PROTEIN-KINASE DURING MITOSIS IN HUMAN-CELLS - CELL-CYCLE DEPENDENT PHOSPHORYLATION AND SUBUNIT REARRANGEMENT [J].

DRAETTA, G ;

BEACH, D .

CELL, 1988, 54 (01) :17-26

[7] CDC2 PROTEIN-KINASE IS COMPLEXED WITH BOTH CYCLIN-A AND CYCLIN-B - EVIDENCE FOR PROTEOLYTIC INACTIVATION OF MPF [J].

DRAETTA, G ;

LUCA, F ;

WESTENDORF, J ;

BRIZUELA, L ;

RUDERMAN, J ;

BEACH, D .

CELL, 1989, 56 (05) :829-838

[8] ASSOCIATION OF HUMAN CYCLIN-E WITH A PERIODIC G(1)-S PHASE PROTEIN-KINASE [J].

DULIC, V ;

LEES, E ;

REED, SI .

SCIENCE, 1992, 257 (5078) :1958-1961

[9] OXY-RADICAL METABOLISM AND CONTROL OF TUMOR-GROWTH [J].

GALEOTTI, T ;

MASOTTI, L ;

BORRELLO, S ;

CASALI, E .

XENOBIOTICA, 1991, 21 (08) :1041-1051

[10]

GESMUNDO N, 1994, BIOCHEM MOL BIOL INT, V33, P705