PHOSPHORYLATION OF PYRUVATE-DEHYDROGENASE IN THE HIPPOCAMPAL SLICE - TIME COURSE OF RESPONSE TO CELLULAR DEPOLARIZATION

被引：2

作者：

CAIN, ST ^{[1
]}

ROUTTENBERG, A ^{[1
]}

机构：

[1] NORTHWESTERN UNIV,CRESAP NEUROSCI LAB,EVANSTON,IL 60201

来源：

NEUROSCIENCE LETTERS | 1991年 / 130卷 / 01期

关键词：

HIPPOCAMPAL SLICE; PROTEIN PHOSPHORYLATION; DEPOLARIZATION; PYRUVATE DEHYDROGENASE COMPLEX; BACK-TITRATION;

D O I：

10.1016/0304-3940(91)90228-L

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In the rat hippocampal slice, we evaluated the effects of potassium depolarization on phosphorylation of the alpha subunit of the pyruvate dehydrogenase complex (E1-alpha, mol. wt. approximately 41 kDa) using an in vitro back-titration assay. The in vitro back-titration phosphorylation of E1-alpha was maximal following 30 s of depolarization and remained elevated for up to 3 min following termination of the depolarizing pulse. Possible significance of and mechanisms for the depolarization-induced changes in E-1-alpha phosphorylation are discussed.

引用

页码：65 / 68

页数：4

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