PREPARATIVE IN-SITU HYBRIDIZATION - SELECTION OF CHROMOSOME REGION-SPECIFIC LIBRARIES ON MITOTIC CHROMOSOMES

被引：18

作者：

HOZIER, J ^{[1
]}

GRAHAM, R ^{[1
]}

WESTFALL, T ^{[1
]}

SIEBERT, P ^{[1
]}

DAVIS, L ^{[1
]}

机构：

[1] CLONTECH LABS INC,PALO ALTO,CA 94303

来源：

GENOMICS | 1994年 / 19卷 / 03期

关键词：

D O I：

10.1006/geno.1994.1092

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed preparative in situ hybridization (Prep-ISH) of complex DNA populations to mitotic chromosomes as a means of generating chromosome region-specific DNA subpopulations. Prep-ISH is a combination of two cytogenetic techniques: in situ hybridization of DNA molecules to mitotic chromosomes and chromosome microdissection. Here, we present test cases demonstrating the feasibility of this approach on mouse and human genomes, using Single nuclei, single chromosomes, or single chromosomal subregions to assess sensitivity, specificity, and representation of the Prep-ISH technique. Prep-ISH has a number of applications in studies of gene expression and genome organization, including efficient cytogenetic sorting of tissue-specific cDNAs and genomic DNA libraries. In addition, Prep-ISH is likely to dramatically reduce the number of candidate genes to aid in gene discovery efforts and to improve efficiency of developing transcription maps and YAC and cosmid contigs through defined cytogenetic regions. (C) 1994 Academic Press, Inc.

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页码：441 / 447

页数：7

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