SIZING OF THE RHODOCOCCUS SP R312 GENOME BY PULSED-FIELD GEL-ELECTROPHORESIS - LOCALIZATION OF GENES INVOLVED IN NITRILE DEGRADATION

被引：9

作者：

BIGEY, F

JANBON, G

ARNAUD, A

GALZY, P

机构：

[1] Chaire de Microbiologie Industrielle et de Génétique des Micro-organismes, E.N.S.A. / I.N.R.A. 2, Montpellier Cedex 1, 34060, Place Viala

来源：

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 1995年 / 68卷 / 02期

关键词：

AMIDASE; GENOME SIZE; NITRILE HYDRATASE; PULSED-FIELD GEL ELECTROPHORESIS; RHODOCOCCUS;

D O I：

10.1007/BF00873103

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The two restriction enzymes AsnI and DraI were found to produce DNA fragment sizes that could be used for mapping the Rhodococcus sp. R312 (formerly Brevibacterium sp. R312) genome by pulsed-field gel electrophoresis. AsnI produced 24 fragments (4 to 727 kb) and DraI yielded 15 fragments (8.5 to 2400 kb). The fragment lengths in each digest were summed, indicating that the size of the chromosome ranged from 6.31 to 6.56 Mb, with a mean of 6.44 Mb. In addition, the wide-spectrum amidase gene (amiE) and the operon containing the enantiomer-selective amidase gene (amdA) and the nitrile hydratase structural gene (nthA, nthB) were localized on the AsnI and DraI fragments.

引用

页码：173 / 179

页数：7

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