Fingerprint Analysis of Codonopsis Radix by HPLC Coupled with Chemometrics Analysis

被引：18

作者：

Chen Fang-fang ^{[1
,2
,3
]}

Qi Huan-yang ^{[1
,2
]}

Shi Yan-ping ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Gansu, Peoples R China

[2] Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Gansu, Peoples R China

[3] Grad Univ, Chinese Acad Sci, Beijing 100039, Peoples R China

来源：

CHINESE HERBAL MEDICINES | 2013年 / 5卷 / 04期

基金：

中国国家自然科学基金;

关键词：

chemometrics; Codonopsis Radix; fingerprinting analysis; HPLC; quality control;

D O I：

10.1016/S1674-6384(13)60046-5

中图分类号：

R914 [药物化学];

学科分类号：

100701 [药物化学];

摘要：

Objective To establish a validated high performance liquid chromatography (HPLC) for the fingerprint analysis of Codonopsis Radix and for the determination of lobetyolin. Methods HPLC coupled with diode array detection method was employed to establish the fingerprint profile and quantitative determination of lobetyolin in Codonopsis Radix. Principal component analysis method was employed to analyze the 52 Codonopsis Radix samples. Results The reference chromatogram was generated with 25 common peaks showing good separation from adjacent peaks. Conclusion Statistical analysis of the obtained data demonstrates that the developed HPLC fingerprint combined with chemometric is a reliable method for the similar evaluation and quality assessment of Codonopsis Radix, and other traditional Chinese herbs.

引用

页码：307 / 312

页数：6

共 24 条

[1]

Dangshen (Codonopsis pilosula) Activates IGF-I and FGF-2 Pathways to Induce Proliferation and Migration Effects in RSC96 Schwann Cells [J].