EFFECTS OF AUTONOMIC NERVOUS SYSTEM-RELATED AGENTS ON THE INTRAVESICAL PRESSURE OF THE BLADDER INSITU IN FEMALE RATS AND AGING

We investigated the effects of several autonomic nervous system-related agents on the intravesical pressure (IVP) in adult (11-23 weeks old, 200-350 g) and aged (2 years old, 350-770 g) female rats. Acetylcholine induced a dose-dependent and transient increase of IVP, which was competitively antagonized by pirenzepine weakly and by atropine strongly. These results suggest the predominance of M-2 receptors. Adrenaline induced dual actions of decrease and increase of IVP at low and high doses, respectively. Adrenaline (at only high doses), noradrenaline and phenylephrine increased IVP but not clonidine, suggesting the predominance of alpha-1 receptors. Isoproterenol, salbutamol and clenbuterol decreased IVP to same extent and the effect of isoproterenol was markedly antagonized by propranolol and slightly by atenolol, suggesting the predominance of beta-2 receptors. ATP increased IVP dose-dependently but not adenosine, suggesting the predominance of P-2 receptors. Serotonin and prostaglandin F2 alpha also increased IVP. In aged rats, the maximal response to acetylcholine was lower than in adult rats and the decrease in IVP by low doses of adrenaline was not observed. These results suggest that the increase of IVP involves the participation of cholinergic M-2 receptors to a large extent and also serotonergic, adrenergic alpha-1 and purinergic P-2 receptors to some extent and that the responsiveness to acetylcholine is reduced by ageing.