INFLUENCE OF PRIMARY AND SECONDARY PROTEASES PRODUCED BY FREE OR IMMOBILIZED CELLS OF THE WHITE-ROT FUNGUS PHANEROCHAETE-CHRYSOSPORIUM ON LIGNIN PEROXIDASE-ACTIVITY

被引：22

作者：

BONNARME, P ^{[1
]}

ASTHER, M ^{[1
]}

机构：

[1] FAC SCI LUMINY,INST NATL RECH AGRON,BIOTECHNOL CHAMPIGNONS FILAMENTEUX LAB,F-13288 MARSEILLE 9,FRANCE

来源：

JOURNAL OF BIOTECHNOLOGY | 1993年 / 30卷 / 03期

关键词：

PROTEASE; LIGNIN PEROXIDASE; DECAY; INACTIVATION; PHANEROCHAETE-CHRYSOSPORIUM; ENZYME PRODUCTION; FREE AND IMMOBILIZED CELLS;

D O I：

10.1016/0168-1656(93)90144-C

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Primary and secondary extracellular proteases produced by free or immobilized cells of the white-rot fungus Phanerochaete chrysosporium have been studied in relation to lignin peroxidase (LiP) decay. Proteases produced during primary metabolism exhibited a maximum activity on day 2; they could totally inactivate LiP activity and partially fragment LiP. Proteases produced during secondary metabolism did not inactivate or decay LiP. These proteases most likely are aspartic- and thiol-proteases.

引用

页码：271 / 282

页数：12

共 26 条

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Dunn BM., 1989, PROTEOLYTIC ENZYMES, P57

[12]

ERIKSSON KE, 1982, EUR J BIOCHEM, V124, P635

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