CARRIER-MEDIATED ACETATE UPTAKE IN CORYNEBACTERIUM-GLUTAMICUM

被引：19

作者：

EBBIGHAUSEN, H ^{[1
]}

WEIL, B ^{[1
]}

KRAMER, R ^{[1
]}

机构：

[1] FORSCHUNGSZENTRUM JULICH,INST BIOTECHNOL,POSTFACH 1913,W-5170 JULICH,GERMANY

来源：

ARCHIVES OF MICROBIOLOGY | 1991年 / 155卷 / 05期

关键词：

ACETATE TRANSPORT-UPTAKE; CORYNEBACTERIUM-GLUTAMICUM; SECONDARY TRANSPORT;

D O I：

10.1007/BF00244970

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Acetate is effectively taken up by whole cells of Corynebacterium glutamicum via a specific carrier with a pH optimum of 8. The K(m) of acetate uptake was 50-mu-M and the V(max) 25-35 nmol/mg dw min. The activation energy was determined to be 70 kJ/mol. Acetate uptake was competitively inhibited by propionate with a K(i) of about 30-mu-M and blocked by addition of sulfhydryl reagents. The transport activity was clearly dependent on the membrane potential, but independent of the presence of Na+-ions. It is concluded that uptake of acetate proceeds by a secondary, proton coupled mechanism.

引用

页码：505 / 510

页数：6

共 39 条

[11]

HARTMANIS MGN, 1984, APPL MICROBIOL BIOT, V20, P66

[12]

HOFER M, 1977, TRANSPORT DURCH BIOL, P68

[13] KINETICS OF SULFATE AND ACETATE UPTAKE BY DESULFOBACTER-POSTGATEI [J].

INGVORSEN, K ;

ZEHNDER, AJB ;

JORGENSEN, BB .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 47 (02) :403-408

[14] FUSION OF PROTOPLASTS AND GENETIC-RECOMBINATION OF BREVIBACTERIUM-FLAVUM [J].

KANEKO, H ;

SAKAGUCHI, K .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1979, 43 (05) :1007-1013

[15]

KARZANOV V V, 1982, Mikrobiologiya, V51, P751

[16] GENETIC-CONTROL OF METABOLISM OF PROPIONATE BY ESCHERICHIA-COLI K12 [J].

KAY, WW .

BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 264 (03) :508-+

[17]

KAY WW, 1978, BACTERIAL TRANSPORT, P385

[18] ON THE PERMEABILITY TO WEAK ACIDS AND BASES OF THE CYTOPLASMIC MEMBRANE OF CLOSTRIDIUM-PASTEURIANUM [J].

KELL, DB ;

PECK, MW ;

RODGER, G ;

MORRIS, JG .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1981, 99 (01) :81-88

[19]

KINOSHITA S, 1985, BIOL IND MICROORGANI, P114

[20]

KLINGENBERG M, 1967, METHOD ENZYMOL, V10, P680

← 1 2 3 4 →