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SYNTHESIS AND BIOPHYSICAL AND BIOLOGICAL PROPERTIES OF OLIGONUCLEOTIDES CONTAINING 2-AZA-2'-DEOXYINOSINE

被引：14
作者：
ACEDO, M
DECLERCQ, E
ERITJA, R
机构：
[1] CSIC,CID,DEPT MOLEC GENET,E-08034 BARCELONA,SPAIN
[2] CATHOLIC UNIV LEUVEN,REGA INST MED RES,B-3000 LOUVAIN,BELGIUM
[3] EUROPEAN MOLEC BIOL LAB,D-69117 HEIDELBERG,GERMANY
来源：
JOURNAL OF ORGANIC CHEMISTRY | 1995年 / 60卷 / 20期
关键词：
D O I：
10.1021/jo00125a009
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
The preparation of oligonucleotides containing 2-aza-2'-deoxyinosine is described. Protection of the 2-azahypoxanthine moiety with the photolabile 2-nitrobenzyl group enabled us to obtain the phosphoramidite derivative and oligonucleotides containing protected 2-aza-2'-deoxyinosine. After purification, photolysis of the oligonucleotides containing the protected analogue provided the desired oligonucleotides in good yields. Melting curves of duplexes containing 2-azahypoxanthine paired with the four natural bases at pH 6 and pH 8 proved that 2-azahypoxanthine base pairs were less stable than perfectly matched duplexes but showed little variation among different bases.
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页码：6262 / 6269
页数：8
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