DETERMINATION OF VINORELBINE IN RABBIT PLASMA BY HIGHPERFORMANCE LIQUID-CHROMATOGRAPHY WITH COULOMETRIC DETECTION

被引:20
作者
MOUCHARDDELMAS, C [1 ]
GOURDIER, B [1 ]
VISTELLE, R [1 ]
机构
[1] CHR REIMS,HOP ROBERT DEBRE,CENT PHARM,PHARM CLIN LAB,F-51096 REIMS,FRANCE
来源
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 663卷 / 02期
关键词
D O I
10.1016/0378-4347(94)00451-A
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A high-performance Liquid chromatographic method was developed for the determination in plasma (400-mu l sample) of a vinca alkaloid, vinorelbine. The analysis was performed by using an octadecylsilane column and heptanesulfonic acid as ion-pairing agent. This method used a new internal standard, teniposide, that permitted a good compromise between sensitivity and retention times (10.6 and 15.5 min for teniposide and vinorelbine, respectively). After a liquid-liquid extraction with diethyl ether, the extracts were injected into a reversed-phase system. The extraction efficiency was approximately 80% for both vinorelbine and the internal standard. The mobile phase was phosphate buffer (pH 3)-acetonitrile-methanol (50:30:20, v/v/v). Using coulometric detection, the limit of detection in plasma (400 mu l) was 1 ng/ml. The intra-assay coefficients of variation were 10.95, 3.80 and 5.71% for 5, 500 and 1000 ng/ml, respectively, and the inter-assay coefficients of variation were 20.14, 14.27 and 10.67% for 5, 500 and 1000 ng/ml, respectively. A linear response was observed for the plasma calibration graph in the ranges 2.5-50 and 50-1000 ng/ml. This method was used to follow the time course of the concentration of vinorelbine in rabbit plasma after a single intravenous dose of vinorelbine (30 mg/m(2)) and seems to be suitable for studying the pharmacokinetics of vinorelbine in rabbit.
引用
收藏
页码:390 / 394
页数:5
相关论文
共 11 条
[1]  
BLANCHARD J, 1981, J CHROMATOGR, V226, P445
[2]   PHARMACOKINETICS OF A NEW ANTICANCER DRUG, NAVELBINE, IN PATIENTS - COMPARATIVE-STUDY OF RADIOIMMUNOLOGIC AND RADIOACTIVE DETERMINATION METHODS [J].
BORE, P ;
RAHMANI, R ;
VANCANTFORT, J ;
FOCAN, C ;
CANO, JP .
CANCER CHEMOTHERAPY AND PHARMACOLOGY, 1989, 23 (04) :247-251
[3]  
CROS S, 1989, SEMIN ONCOL, V16, P15
[4]   DETERMINATION OF VINORELBINE (NAVELBINE) IN TUMOR-CELLS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
DEBAL, V ;
MORJANI, H ;
MILLOT, JM ;
ANGIBOUST, JF ;
GOURDIER, B ;
MANFAIT, M .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 581 (01) :93-99
[5]  
DEPIERRE A, 1989, SEMIN ONCOL S4, V16, P30
[6]   PHASE-II TRIAL OF WEEKLY INTRAVENOUS VINORELBINE IN 1ST-LINE ADVANCED BREAST-CANCER CHEMOTHERAPY [J].
FUMOLEAU, P ;
DELGADO, FM ;
DELOZIER, T ;
MONNIER, A ;
DELGADO, MAG ;
KERBRAT, P ;
GARCIAGIRALT, E ;
KEILING, R ;
NAMER, M ;
CLOSON, MT ;
GOUDIER, MJ ;
CHOLLET, P ;
LECOURT, L ;
MONTCUQUET, P .
JOURNAL OF CLINICAL ONCOLOGY, 1993, 11 (07) :1245-1252
[7]   DETERMINATION OF NAVELBINE AND DESACETYLNAVELBINE IN BIOLOGICAL-FLUIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
JEHL, F ;
DEBS, J ;
HERLIN, C ;
QUOIX, E ;
GALLION, C ;
MONTEIL, H .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 525 (01) :225-233
[8]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF NAVELBINE IN HUMAN PLASMA AND URINE [J].
NICOT, G ;
LACHATRE, G ;
MARQUET, P ;
BONNAUD, F ;
VALLETTE, JP ;
ROCCA, JL .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 528 (01) :258-266
[9]  
RAHMANI R, 1984, CANCER RES, V44, P5609
[10]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF NAVELBINE IN MO4 MOUSE FIBROSARCOMA CELLS AND BIOLOGICAL-FLUIDS [J].
VANBELLE, SJP ;
DESMET, M ;
MONSAERT, C ;
GEERTS, F ;
STORME, GA ;
MASSART, DL .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 576 (02) :351-357