ISOLATION AND CHARACTERIZATION OF METHANOBREVIBACTER-ORALIS SP-NOV

被引：104

作者：

FERRARI, A ^{[1
]}

BRUSA, T ^{[1
]}

RUTILI, A ^{[1
]}

CANZI, E ^{[1
]}

BIAVATI, B ^{[1
]}

机构：

[1] UNIV BOLOGNA,INST AGR MICROBIOL,BOLOGNA,ITALY

来源：

CURRENT MICROBIOLOGY | 1994年 / 29卷 / 01期

关键词：

D O I：

10.1007/BF01570184

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A new coccobacillary, nonmotile, Gram-positive, methane-producing organism was isolated from human subgingival plaque. Both hydrogen and carbon dioxide were required for growth. No methane was produced from acetate, formate, or methanol. The optimum pH was 6.9-7.4, and the optimum temperature was 36-38 degrees C. Fecal extract was required for growth, and a volatile fatty acid mixture was highly stimulatory. The DNA G+C content was 28 mol%. On the basis of these characteristics, DNA-DNA hybridization studies, and electrophoretic analysis of cellular proteins, the isolate was considered a new species and named Methanobrevibacter oralis.

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页码：7 / 12

页数：6

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共 24 条

[11] PROCEDURE FOR ISOLATION OF DEOXYRIBONUCLEIC ACID FROM MICRO-ORGANISMS [J].

MARMUR, J .

JOURNAL OF MOLECULAR BIOLOGY, 1961, 3 (02) :208-&

[12] DETERMINATION OF BASE COMPOSITION OF DEOXYRIBONUCLEIC ACID FROM ITS THERMAL DENATURATION TEMPERATURE [J].

MARMUR, J ;

DOTY, P .

JOURNAL OF MOLECULAR BIOLOGY, 1962, 5 (01) :109-&

[13] METHANOSPHAERA-STADTMANIAE GEN-NOV, SP-NOV - A SPECIES THAT FORMS METHANE BY REDUCING METHANOL WITH HYDROGEN [J].

MILLER, TL ;

WOLIN, MJ .

ARCHIVES OF MICROBIOLOGY, 1985, 141 (02) :116-122

[14] SERUM BOTTLE MODIFICATION OF HUNGATE TECHNIQUE FOR CULTIVATING OBLIGATE ANAEROBES [J].

MILLER, TL ;

WOLIN, MJ .

APPLIED MICROBIOLOGY, 1974, 27 (05) :985-987

[15] ENUMERATION OF METHANOBREVIBACTER-SMITHII IN HUMAN FECES [J].

MILLER, TL ;

WOLIN, MJ .

ARCHIVES OF MICROBIOLOGY, 1982, 131 (01) :14-18

[16] ISOLATION OF METHANOBREVIBACTER-SMITHII FROM HUMAN FECES [J].

MILLER, TL ;

WOLIN, MJ ;

DEMACARIO, EC ;

MACARIO, AJL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1982, 43 (01) :227-232

[17] POLYACRYLAMIDE SLAB GEL-ELECTROPHORESIS OF SOLUBLE-PROTEINS FOR STUDIES OF BACTERIAL FLORAS [J].

MOORE, WEC ;

HASH, DE ;

HOLDEMAN, LV ;

CATO, EP .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1980, 39 (04) :900-907

[18] USE OF LEAD CITRATE AT HIGH PH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY [J].

REYNOLDS, ES .

JOURNAL OF CELL BIOLOGY, 1963, 17 (01) :208-&

[19] ISOLATION AND CHARACTERIZATION OF METHANOBACTERIUM-RUMINANTIUM N-SP [J].

SMITH, PH ;

HUNGATE, RE .

JOURNAL OF BACTERIOLOGY, 1958, 75 (06) :713-718

[20] COENZYME-M, ESSENTIAL FOR GROWTH OF A RUMEN STRAIN OF METHANOBACTERIUM-RUMINANTIUM [J].

TAYLOR, CD ;

MCBRIDE, BC ;

WOLFE, RS ;

BRYANT, MP .

JOURNAL OF BACTERIOLOGY, 1974, 120 (02) :974-975