ISOLATION AND CHARACTERIZATION OF METHANOBREVIBACTER-ORALIS SP-NOV

被引：104

作者：

FERRARI, A ^{[1
]}

BRUSA, T ^{[1
]}

RUTILI, A ^{[1
]}

CANZI, E ^{[1
]}

BIAVATI, B ^{[1
]}

机构：

[1] UNIV BOLOGNA,INST AGR MICROBIOL,BOLOGNA,ITALY

来源：

CURRENT MICROBIOLOGY | 1994年 / 29卷 / 01期

关键词：

D O I：

10.1007/BF01570184

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A new coccobacillary, nonmotile, Gram-positive, methane-producing organism was isolated from human subgingival plaque. Both hydrogen and carbon dioxide were required for growth. No methane was produced from acetate, formate, or methanol. The optimum pH was 6.9-7.4, and the optimum temperature was 36-38 degrees C. Fecal extract was required for growth, and a volatile fatty acid mixture was highly stimulatory. The DNA G+C content was 28 mol%. On the basis of these characteristics, DNA-DNA hybridization studies, and electrophoretic analysis of cellular proteins, the isolate was considered a new species and named Methanobrevibacter oralis.

引用

页码：7 / 12

页数：6