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ON-COLUMN AND POSTCOLUMN DERIVATIZATION FOR CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE FOR THE ANALYSIS OF SINGLE CELLS

被引：36
作者：
HIETPAS, PB
EWING, AG
机构：
[1] Department of Chemistry, Penn State University, University Park, Pennsylvania, 16802
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY | 1995年 / 18卷 / 18-19期
基金：
美国国家科学基金会; 美国国家卫生研究院;
关键词：
D O I：
10.1080/10826079508014611
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Derivatization methods for analysis of single cell components by capillary electrophoresis with laser-induced fluorescence detection are reviewed. Recent advances have lead to the use of this technique for the identification of amino acids and proteins in many different cell lines ranging in size from 8 to 140 mu m. Pre-column, on-column, and post-column methods of derivatization are examined in order to determine which results in the least amount of sample handling and dilution leading to better detection limits for the analytes of interest.
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页码：3557 / 3576
页数：20
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