DETERMINATION OF THE PATTERN OF METHYL ESTERIFICATION IN PECTIN - DISTRIBUTION OF CONTIGUOUS NONESTERIFIED RESIDUES

被引：84

作者：

MORT, AJ ^{[1
]}

QIU, F ^{[1
]}

MANESS, NO ^{[1
]}

机构：

[1] OKLAHOMA STATE UNIV,DEPT HORT & LANDSCAPE ARCHITECTURE,STILLWATER,OK 74078

来源：

CARBOHYDRATE RESEARCH | 1993年 / 247卷

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0008-6215(93)84238-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for determining the distribution of contiguous nonesterified galacturonic acid residues within pectins. First, the esterified galacturonic acids are converted to galactose by reduction with sodium borohydride, then the glycosidic linkages of the resulting galactose residues are cleaved selectively by liquid HF solvolysis. Separation and quantitation of the resulting galacturonic acid containing oligomers reveals the proportion of each stretch of contiguous nonesterified galacturonic acid residues in the original pectin. The distribution of nonesterified GalA in a pectin fraction obtained from cotton suspension culture cell walls with approximately 50% esterification appears to be far from random.

引用

页码：21 / 35

页数：15

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