DNA-FINGERPRINTING OF YEAST STRAINS BY RESTRICTION ENZYME ANALYSIS

被引：9

作者：

BARBERIO, C

FANI, R

RASO, A

CARLI, A

POLSINELLI, M

机构：

[1] Dipartimento di Biologia Animale e Genetica, Universitá di Firenze, 50125 Firenze

来源：

RESEARCH IN MICROBIOLOGY | 1994年 / 145卷 / 09期

关键词：

YEAST; DNA FINGERPRINTING; RESTRICTION ENZYME ANALYSIS; DIAGNOSIS; TAXONOMY;

D O I：

10.1016/0923-2508(94)90037-X

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Restriction endonuclease analysis was used as a new method to obtain genomic DNA fingerprints in yeast. Fifteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces were examined. Restriction fragments obtained with ApaI or KspI endonucleases were separated by SDS-PAGE and silver-stained. Analysis of the fingerprints showed that restriction enzyme digestion of genomic DNA can be successfully applied to yeast, enabling the differentiation between strains belonging to different or to the same species or genera.

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页码：659 / 666

页数：8

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