EVALUATION OF E-TEST FOR DETERMINATION OF ANTIMICROBIAL MICS FOR PSEUDOMONAS-AERUGINOSA ISOLATES FROM CYSTIC-FIBROSIS PATIENTS

被引:19
作者
MARLEY, EF
MOHLA, C
CAMPOS, JM
机构
[1] CHILDRENS NATL MED CTR, DEPT LAB MED, WASHINGTON, DC 20010 USA
[2] GEORGE WASHINGTON UNIV, MED CTR, DEPT PATHOL, WASHINGTON, DC 20037 USA
[3] GEORGE WASHINGTON UNIV, MED CTR, DEPT PEDIAT, WASHINGTON, DC 20037 USA
[4] GEORGE WASHINGTON UNIV, MED CTR, DEPT MICROBIOL, WASHINGTON, DC 20037 USA
[5] CHILDRENS NATL MED CTR, DEPT LAB MED, WASHINGTON, DC 20010 USA
关键词
D O I
10.1128/JCM.33.12.3191-3193.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We determined the E-Test and National Committee for Clinical Laboratory Standards standardized agar dilution MICs of ceftazidime, ciprofloxacin, piperacillin, and tobramycin for Pseudomonas aeruginosa during tests of 100 rough and mucoid P. aeruginosa isolates from cystic fibrosis patients, The levels of agreement (+/-1 log, dilution) between quantitative E-Test and agar dilution MIC results were 80, 97, 73, and 89% for ceftazidime, ciprofloxacin, piperacillin, and tobramycin, respectively, Comparison of the results after converting the MIC data to qualitative categories (susceptible, intermediate, and resistant) yielded levels of agreement of 84, 96, 88, and 93% for the same agents, respectively. Of the 39 qualitative discrepancies, 36 were minor and 3 were very major. We conclude that use of the E-Test is easier and more practical than use of the agar dilution method for most laboratories and that the E-Test furnishes results which are at least as accurate as those obtained by the agar dilution method, However, the higher cost of the E-Test method would likely discourage most laboratories from selecting it over disk diffusion for routine antimicrobial susceptibility testing of P. aeruginosa isolates from cystic fibrosis patients.
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页码:3191 / 3193
页数:3
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