DETERMINATION OF DEHYDROASCORBIC ACID BY AN ENZYME SENSOR SYSTEM WITH A PARALLEL-FLOW CELL

被引：7

作者：

ENDO, H

MATSUNAGA, C

HOSHI, M

HAYASHI, T

TAKAI, R

WATANABE, E

机构：

[1] TOKYO UNIV FISHERIES,DEPT FOOD SCI & TECHNOL,KONAN 4,MINATO KU,TOKYO 108,JAPAN

[2] MARUHA CORP,CENT RES INST,TSUKUBA,IBARAKI 30042,JAPAN

来源：

BIOSENSORS & BIOELECTRONICS | 1994年 / 9卷 / 07期

关键词：

BIOSENSOR; ENZYME SENSOR; DEHYDROASCORBIC ACID; L-ASCORBIC ACID; DETERMINATION;

D O I：

10.1016/0956-5663(94)90012-4

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An ezyme sensor system was developed for the determination of dehydroascorbic acid and L-ascorbic acid. The system was constructed from a parallel flow cell separated by a dialysis membrane, an immobilized ascorbate oxidase membrane, coupled to a Clark-type oxygen electrode, and L-cysteine, which was present as a reductant. The method is based on the reduction of dehydroascorbic acid to L-ascorbic acid with L-cysteine in a parallel flow cell. One assay for dehydroascorbic acid could be completed within 8 min. Linear calibratin curves for dehydroascorbic acid and L-ascorbic and were observed in the range of 20 mM-100 mM and 2 mM-20 mM, respectively.

引用

页码：501 / 507

页数：7

共 12 条

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