AUTOCRINE RECEPTORS FOR ENDOTHELINS IN THE PRIMARY CULTURE OF ENDOTHELIAL-CELLS OF HUMAN UMBILICAL VEIN

被引：67

作者：

FUJITANI, Y ^{[1
]}

ODA, K ^{[1
]}

TAKIMOTO, M ^{[1
]}

INUI, T ^{[1
]}

OKADA, T ^{[1
]}

URADE, Y ^{[1
]}

机构：

[1] CIBA GEIGY JAPAN LTD,INT RES LABS,10-66 MIYUKI CHO,TAKARAZUKA 665,JAPAN

来源：

FEBS LETTERS | 1992年 / 298卷 / 01期

关键词：

ENDOTHELIN; ENDOTHELIAL CELL; RECEPTOR; VASODILATION;

D O I：

10.1016/0014-5793(92)80026-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human umbilical vein endothelial cells (HUVECs) in primary culture produced and secreted endothelin 1 (ET-1) actively. Specific binding of [I-125]ET-1 to these cells was not detectable because of the saturation of ET receptors with endogenously produced ET-1. However, addition of phosphoramidon, an inhibitor of ET-converting enzyme. to the medium reduced the production of ET-1 and thus the receptors on HUVECs were made available for exogenously added [I-125]ET-1. Binding studies using phosphoramidon-treated HUVECs indicated the existence of a non-isopeptide-selective type (ET(B)) of ET receptor with a K(d) of 17 pM. This receptor is thought to be involved in ET-induced vasodilation in an autocrine manner in vivo.

引用

页码：79 / 83

页数：5

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