CLONING OF THE HUMAN HOMOLOG OF THE CDC34 CELL-CYCLE GENE BY COMPLEMENTATION IN YEAST

被引：90

作者：

PLON, SE

LEPPIG, KA

DO, HN

GROUDINE, M

机构：

[1] FRED HUTCHINSON CANC RES CTR,SEATTLE,WA 98104

[2] UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98195

[3] UNIV WASHINGTON,DEPT PATHOL,SEATTLE,WA 98195

[4] UNIV WASHINGTON,DEPT RADIAT ONCOL,SEATTLE,WA 98195

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 22期

关键词：

D O I：

10.1073/pnas.90.22.10484

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In a screen designed to isolate human cDNAs that complement a yeast G2 phase checkpoint mutation (mec1), we isolated a cDNA homologous to the Saccharomyces cerevisiae CDC34 gene. The human CDC34 cDNA can functionally substitute for the yeast CDC34 gene and represents a mammalian homolog of the group of yeast genes required for the late G1 --> S phase transition. The human CDC34 gene is expressed in multiple cell lines as a unique species and Southern blot analysis reveals evidence for a single gene that is highly conserved in higher eukaryotes. The human gene is located on the far telomeric region of 19p13.3 in a location that defines a region of homology between human chromosome 19p and mouse chromosome 11.

引用

页码：10484 / 10488

页数：5