CA2+/MG2+-DEPENDENT ENDONUCLEASE ACTIVATION IS AN EARLY EVENT IN VP-16-INDUCED APOPTOSIS OF HUMAN BREAST-CANCER MCF7 CELLS IN-VITRO

被引：37

作者：

SOKOLOVA, IA

COWAN, KH

SCHNEIDER, E

机构：

[1] Medicine Branch, National Cancer Institute, Bethesda, Maryland

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1995年 / 1266卷 / 02期

关键词：

ENDONUCLEASE; APOPTOSIS; DNA FRAGMENTATION;

D O I：

10.1016/0167-4889(94)00233-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Apoptosis is now recognized as one of the major processes regulating the size of cell populations. However, despite intensive investigations the biochemical and enzymological mechanisms involved in apoptosis remain unclear. In the present study we demonstrate activation of a Ca2+/Mg2+-dependent endonuclease during VP-16-induced apoptosis in MCF7 cells. Nuclease activation occurred prior to the appearance of internucleosomal DNA fragmentation, suggesting that this activation may be an early and possibly critical step in drug-induced apoptosis. Analysis of the internucleosomal DNA fragments showed that they contained phosphorylated 5'-ends, indicating that they were produced by a Ca2+/Mg2+-dependent endonuclease.

引用

页码：135 / 142

页数：8

共 35 条

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