A CONVENIENT APPROACH TO THE SYNTHESIS OF MEDIUM SIZE OLIGODEOXYRIBONUCLEOTIDES BY IMPROVED NEW PHOSPHITE METHOD

被引：18

作者：

HOSAKA, H ^{[1
]}

SUZUKI, Y ^{[1
]}

SATO, H ^{[1
]}

GUGKIM, S ^{[1
]}

TAKAKU, H ^{[1
]}

机构：

[1] CHIBA INST TECHNOL,DEPT IND CHEM,NARASHINO,CHIBA 275,JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 11期

关键词：

D O I：

10.1093/nar/19.11.2935

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Improvement of the new phosphite method for the synthesis of oligodeoxyribonucleotides using the deoxyribonucleoside 3'-bis(1,1,1,3,3,3- hexafluoro-2-propyl) phosphite unit has been carried out via the hydrolysis and capping steps, without any side reaction products. The new phosphite unit and capping agent, bis(1,1,1,3,3,3-hexafluoro-2-propyl)-2-propyl phosphite, is readily activated by N-methylimdazole under very mild condition on a solid support. This operation involves a one pot reaction, which is an advantage over both the phosphite and H- phosphonate approaches. The mechanism of internucleotidic bond formation of the new phosphite method is also discussed.

引用

页码：2935 / 2940

页数：6

共 29 条

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