EFFICIENT PRODUCTION OF ANTIBODY FRAGMENTS BY THE FILAMENTOUS FUNGUS TRICHODERMA-REESEI

被引：81

作者：

NYYSSONEN, E

PENTTILA, M

HARKKI, A

SALOHEIMO, A

KNOWLES, JKC

KERANEN, S

机构：

[1] VTT BIOTECH LAB, POB 202, SF-02151 ESPOO, FINLAND

[2] GLAXO INST MOLEC BIOL, CH-1228 PLAN LES OUATES, SWITZERLAND

来源：

BIO-TECHNOLOGY | 1993年 / 11卷 / 05期

关键词：

D O I：

10.1038/nbt0593-591

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have engineered the filamentous fungus Trichoderma reesei to assemble and secrete immunologically authentic engineered Fab antibody fragments into the culture medium. A major improvement in yield was achieved by fusing the heavy Fd chain to the T. reesei cellulase, CBHI. The yields of secreted, immunologically active Fab and CBHI-Fab fusion were 1 mg/l and 150 mg/l, respectively. The Fab fragment can be released from the fusion protein CBHI-Fab by an extracellular T reesei protease. There was no detectable difference in affinity for the antigen between the engineered Fab and the idiotypic antibody.

引用

页码：591 / 595

页数：5

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