RELATIONSHIPS BETWEEN CELL-SURFACE PROTEASE AND ACID-PHOSPHATASE ACTIVITIES OF LEISHMANIA PROMASTIGOTE

被引：7

作者：

HERNANDEZ, AG

RASCON, A

KUTNER, S

ROMAN, H

CAMPOS, Z

机构：

[1] Departamento de Biologia Celular, Facultad de Ciencias, Universidad Central de Venezuela, Apartado 47069, Los Chaguaramos, Caracas

[2] Department of Parasitology and the Kuvin Centre for the Study of Infectious and Tropical Diseases, The Hebrew University, Jerusalem

来源：

MOLECULAR BIOLOGY REPORTS | 1993年 / 18卷 / 03期

关键词：

LEISHMANIA; CELL SURFACE; PROTEASE; ACID PHOSPHATASE; RADIO-IODINATION;

D O I：

10.1007/BF01674430

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A correlation between the ratio of the cell surface protease activity to phosphatase activity and the complexity of the pattern of cell surface exposed polypeptides of Leishmania promastigotes was demonstrated for various strains grown under similar conditions. The ratio of the cell surface protease activity to acid phosphatase activity was high for L. major and L.b. panamensis and it correlates with the expression of a single polypeptide of 63 kDa on their cell surface. Intermediate and lower ratios of these enzymatic activities relate with more complex radio-iodinated patterns: two main bands in L.b. guyanensis (70 and 58 KDa) and L.b. braziliensis (72 and 60 KDa) and three main bands 65, 50, 27 KDa in all L.m. mexicana strains tested. Evidence is presented that the acid phosphatase located on the L.m. mexicana cell surface is not an artifact due to a secondary absorption of the secreted acid phosphatase from the culture medium. These results confirm the Leishmania antigen cell surface heterogeneity. The implication on the biology of Leishmania and the clinical manifestation of leishmaniasis are discussed.

引用

页码：189 / 195

页数：7

共 32 条

[21]

KWEIDER M, 1987, J IMMUNOL, V138, P299

[22] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[23] COMPARISON OF EXTRACELLULAR ACID-PHOSPHATASES FROM VARIOUS ISOLATES OF LEISHMANIA [J].

LOVELACE, JK ;

GOTTLIEB, M .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1986, 35 (06) :1121-1128

[24] BIOCHEMICAL-EVIDENCE OF THE ANTIGENIC CELL-SURFACE HETEROGENEITY OF LEISHMANIA-MEXICANA [J].

MANENTI, S ;

KUTNER, S ;

RASCON, A ;

HERNANDEZ, AG .

PARASITOLOGY RESEARCH, 1990, 76 (04) :301-305

[25] SURFACE-SPECIFIC IODINATION OF MEMBRANE PROTEINS OF VIRUSES AND EUKARYOTIC CELLS USING 1,3,4,6-TETRACHLORO-3-ALPHA,6-ALPHA-DIPHENYLGLYCOLURIL [J].

MARKWELL, MAK ;

FOX, CF .

BIOCHEMISTRY, 1978, 17 (22) :4807-4817

[26] PURIFICATION AND CHARACTERIZATION OF A MEMBRANE-BOUND ACID-PHOSPHATASE OF LEISHMANIA-MEXICANA [J].

MENZ, B ;

WINTER, G ;

ILG, T ;

LOTTSPEICH, F ;

OVERATH, P .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 47 (01) :101-108

[27] CHEMOTHERAPY OF CUTANEOUS LEISHMANIASIS - LEISHMANIA TROPICA INFECTIONS IN MICE [J].

NEAL, RA .

ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 1964, 58 (04) :420-&

[28]

PIMENTA PFP, 1986, J SUBMICR CYTOL PATH, V18, P127

[29] THE RIBOSOMAL GENE SPACER AS A TOOL FOR THE TAXONOMY OF LEISHMANIA [J].

RAMIREZ, JL ;

GUEVARA, P .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1987, 22 (2-3) :177-183

[30]

REMALEY AT, 1985, J BIOL CHEM, V260, P880

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