HYPERSENSITIVITY TO CHIRONOMIDS (NON-BITING MIDGES) - LOCALIZATION OF THE ANTIGENIC DETERMINANTS WITHIN CERTAIN POLYPEPTIDE SEQUENCES OF HEMOGLOBINS (ERYTHROCRUORINS) OF CHIRONOMUS-THUMMI-THUMMI (DIPTERA)

被引：78

作者：

BAUR, X ^{[1
]}

DEWAIR, M ^{[1
]}

FRUHMANN, G ^{[1
]}

ASCHAUER, H ^{[1
]}

PFLETSCHINGER, J ^{[1
]}

BRAUNITZER, G ^{[1
]}

机构：

[1] MAX PLANCK INST BIOCHEM, D-8033 MARTINSRIED, FED REP GER

来源：

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY | 1982年 / 69卷 / 01期

关键词：

D O I：

10.1016/0091-6749(82)90090-2

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Humans (99) who were in contact with larvae of chironomids were investigated; 43 developed respiratory, conjunctival or cutaneous hypersensitivity reactions upon exposure to airborne constituents of these insects. By RAST [radioallergosorbent test], RAST inhibition and skin tests, type I sensitization to Hb of chironomids was identified in 33 subjects of the symptomatic group. In 1 case additional proof for high-grade sensitization was obtained by bronchial provocation with as low as 0.2 .mu.g of 1 of the isolated Hb. RAST and skin-test investigations with fragments obtained by chemical and enzymatic cleavage of 2 of the homologous Hb led to localization of the antigenic determinants within the peptide sequences 1-31, 32-90, 91-101 and 109-131 of Hb CTT IV and within the sequence 64-98 of Hb CTT VI of C. t. thummi. Hb of chironomids are potent allergens in man. Chironomids must be considered in relation to environmental and occupational sensitization contributing to respiratory diseases.

引用

页码：66 / 76

页数：11

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